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In the healthcare field, hand hygiene is the most important factor in preventing the spread of infections. Although many hand hygiene products are available, healthcare workers usually do not adhere to the manufacturers’ recommendations for use. Improper use of the products decreases their efficacy; therefore methods to assess quality of hand hygiene are necessary. Non-degradable dyes are currently used for monitoring the effectiveness of soap and water, but are not advantageous for monitoring waterless alcohol-based products. The first goal is to identify or develop a product whose fluorescent properties are altered when exposed to alcohol-based hand products. The second goal is to develop a mobile spectrofluorometer that can measure fluorescence on hands in order to quantitatively assess hand hygiene.