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The client desires a method of increasing the pO2 of the digestion media for purifying and transplanting human pancreatic islets of Langerhans in curing type 1 diabetes. The method should avoid aerosols and must not compromise sterility of the islets. Current methods are limited due to technical aspects of the isolation procedure that negatively impact islet viability and function post isolation, including ischemic damage due to the lack of sufficient oxygen tension in the media used to digest the pancreas. The effect of hyperoxygenating the media on islet yield and viability will be measured and compared to isolations performed by the standard method.
Our Team
A mock set up of the actual islet purification apparatus:
Parts for the construction of our prototypes:
Glass was custom blown courtesy of Shane Flickinger. Gas permeable silicone tubing is coiled for oxygen diffusion.
End-cap for plastic prototype with holes for islet media and oxygen.
Islet media flows through the chamber while oxygen diffuses through the tubing. A pO2 sensor would be placed at the outlet.
Prototype is set up in a test circuit and monitored to see if the pO2 concentration increases.
The oxygenation chamber successfully increased the pO2 concentration of the test media.
Our poster during the BME Student Design Expo:
Tim, April, and Megan at the Design Expo. It’s good to be here.
We had the BME Student Design Expo on Dec. 3. It went very well and was lots of fun. We saw many awesome design projects and had a lot of interest in our own. We’ve completed all deliverables and
have finalized details with our Client.
Future Work: Improve design to increase diffusion and make disposable.
Special thanks to Prof. John Webster, Dr. Matthew Hanson, Eric Hatch, Tracy Drier, and Shane Flickinger.
| Week | Reporting Period Beginning | Activities |
|---|---|---|
| 1 | September 3 | form teams; select project; contact client |
| 2 | September 10 | literature search; meet with client |
| 3 | September 17 | develop understanding of project; develop PDS |
| 4 | September 24 | brainstorm |
| 5 | October 1 | work on presentations |
| 6 | October 8 | presentations |
| 7 | October 15 | decide on final design; hand in written report including PDS; submit design notebooks for advisor review |
| 8 | October 22 | work on design |
| 9 | October 29 | work on design |
| 10 | November 5 | work on design |
| 11 | November 12 | work on design |
| 12 | November 19 | work on design |
| 14 | December 3 | FINAL POSTER PRESENTATIONS |
| 15 | December 10 | hand in written report and notebook to advisor; final meetings with advisors |
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Mid-Semester Presentation (Oct 7 2004, 134 kb) |
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Mid-Semester Report (Oct 14 2004, 533 kb) |
|
Final Presentation (Dec 6 2004, 1034 kb) |
|
Final Report (Dec 6 2004, 1015 kb) |
|
Product Design Specifications (Dec 8 2004, 12 kb) |
